The technology of Nitrification Inhibitors dates from the 60s (Nitrapyrin in the USA), then in the 80s with various molecules a certain development in Europe occurred based on the molecule Dicyandiamide (DCD), but it only had a commercially important development starting from 2000 with the introduction of fertilizers using the molecule 3,4 DMPP.
The main objective of these molecules, Nitrification Inhibitors, is to reduce the groundwater contamination with Nitrates. These Nitrates can have origin of Nitrate fertilizers themselves and / or conventional Ammonium fertilizers where the Ammonium of these is quickly transformed to Nitrate by the action of soil bacteria called Nitrosomonas, thus leaving Nitrogen susceptible to being leached, even in Fertigation.
Ammonium is fixed in the clay-humic complex and therefore does not leach. When applying Ammonium fertilizer to the soil, the activity of Nitrosomonas is inhibited / depressed for a certain period of time by using a Nitrification inhibitor such as 3,5 DMPP contained in NITRO BRAKE. The Nitrification process will be interrupted and thus the Ammonium will remain, as such, for a longer time, increasing its assimilation by the plant and thus the efficiency of the applied Ammonium or Urea fertilizer.
The mode of action of DMPP is based on its coupling to the enzyme called AMO (Ammonium Mono Oxygenase), part of the bacteria called Nitrosomonas spp, thereby disabling the enzyme. Without access to this enzyme Nitrosomonas spp. cannot transform Ammonium into Nitrate. Only when AMO enzyme is available again for binding Ammonium the bacteria can start the nitrification process.
DMPP has specific activity on Nitrosomonas, it does not affect other soil bacteria and since it does not kill Nitrosomonas, but only causes its temporarily limited blocking, DMPP is considered to be bacteriostatic and not bactericidal.